The septum transversum begins to condense from mesoderm during the third week of development. It appears in the cervical region and is innervated by cervical spinal nerves. As the embryo folds in the rostral and caudal directions the septum transvernsum is displaced caudally in the embryo but carries its original nerve supply with it. This explains why the phrenic nerve, the main nerve supply to the diaphragm consists of axons from spinal nerves C3, C4, and C5.


In this embryo we are looking up at the inferior surface of the septum transversum, which is located anteriorly in the embryo and which partially separates the developing abdomen from the developing thorax. The esophagus passes posterior to it and the mesentery of the esophagus is connected to it. Pericardioperitoneal canals connect the peritoneal cavity (abdomen) with the thoracic cavity (pleural cavity) on either side of the midline. Completion of the diaphragm is by closure of the pericardioperitoneal canals by the growth of a pair of pleuroperitoneal membranes.